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MCT1/Malignant T cell amplified sequence 1 Rabbit pAb (bs-1569R)  
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產品編號 bs-1569R
英文名稱 MCT1/Malignant T cell amplified sequence 1 Rabbit pAb
中文名稱 單羧酸轉運蛋白-1抗體
別    名 monocarboxylate transporter 1; Malignant T cell amplified sequence 1; MCT 1; MCT1; MCTS 1; MCTS1; Oncogene MCT 1; Oncogene MCT1; SLC16A1; FLJ36745; HHF7; MCT; MGC44475; monocarboxylate transporter; Monocarboxylate transporter isoform 1; Monocarboxylic acid transporter 1; SLC16A1; SLC16A1 protein; Solute carrier family 16(monocarboxylic acid transporters) member 1; Solute carrier family 16 member 1(monocarboxylic acid transporter 1); Solute carrier family 16 member 1; MOT1_HUMAN.  
Specific References  (1)     |     bs-1569R has been referenced in 1 publications.
[IF=0] Klasvogt, Sonja, et al. "Air–liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2." Cell Death Discovery 3 (2017): 17001.  WB ;  Pig.  
研究領域 免疫學  轉運蛋白  交換蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,GuineaPig,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 55 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MCT1: 34-59/500 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009]

Function:
Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Widely expressed in normal and in cancer cells.

DISEASE:
Defects in SLC16A1 are the cause of symptomatic deficiency in lactate transport (SDLT) [MIM:245340]; also known as erythrocyte lactate transporter defect. Deficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals.
Defects in SLC16A1 are the cause of familial hyperinsulinemic hypoglycemia type 7 (HHF7) [MIM:610021]; also known as exercise-induced hyperinsulinemic hypoglycemia. HHF7 is a dominantly inherited hypoglycemic disorder characterized by inappropriate insulin secretion during anaerobic exercise or on pyruvate load.

Similarity:
Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

SWISS:
P53985

Gene ID:
6566

Database links:

Entrez Gene: 6566 Human

Entrez Gene: 20501 Mouse

Omim: 600682 Human

SwissProt: P53985 Human

SwissProt: P53986 Mouse

Unigene: 75231 Human

Unigene: 9086 Mouse



單羧酸轉運蛋白家族是哺乳動物細胞膜上一類重要的跨膜轉運蛋白,負責乳酸、短鏈脂肪酸等單羧酸類化合物的跨膜轉運,有促進營養物質吸收、代謝動態的平衡、調節胞內pH值以及參與藥物輸送等多種生物學功能。
產品圖片
Sample: Brain(Rat) lysate at 45ug; Colon carcinoma(Human) lysate at 45ug; Primary: Anti-MCT1 (bs-1569R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; Predicted band size : 55kD Observed band size : 55kD
Sample: Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Stomach (Mouse) Lysate at 40 ug Lane 3: Heart (Mouse) Lysate at 40 ug Lane 4: Liver (Rat) Lysate at 40 ug Lane 5: Stomach (Rat) Lysate at 40 ug Lane 6: Heart (Rat) Lysate at 40 ug Primary: Anti-MCT1 (bs-1569R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MCT1 Polyclonal Antibody, Unconjugated(bs-1569R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MCT1 Polyclonal Antibody, Unconjugated(bs-1569R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
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