| 產品編號 | bs-46004P |
| 英文名稱 | Recombinant SARS-Cov-2 Spike S1 protein, His & Avi (HEK293) |
| 中文名稱 | 重組SARS冠狀病毒2 Spike S1 蛋白 |
| 別 名 | coronavirus spike Protein; cov spike Protein; novel coronavirus RBD Protein; novel coronavirus s1 Protein; novel coronavirus spike Protein; RBD Protein; S1 Protein; Spike RBD Protein; 2019-nCoV; 2019nCoV; Recombinant SARS-Cov-2 Spike S1 protein, His & Avi (HEK293) 新冠病毒S蛋白; 新冠S1蛋白; SARS-CoV-2 S1蛋白; |
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Specific References (1) | bs-46004P has been referenced in 1 publications.
[IF=4.38] Lalioti, Vasiliki. et al. Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia. SCI REP-UK. 2022 Apr;12(1):1-19 Other ; Other.
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| 克 隆 號 | |
| 理論分子量 | 73 kDa |
| 檢測分子量 | 120-140 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | SARS-Cov-2 |
| 序 列 | 14-683/1273 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | Not analyzed |
| 表達系統 | HEK293 cell |
| 活性 | Yes |
| 標簽 | His & Avi |
| 緩 沖 液 | Lyophilized from 0.22um filtered solution in 2X PBS (pH7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| 保存條件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensinconverting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| 產品圖片 |
Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: bs-46001P) at 2μg/ml (100ul/Well) can bind SARS-CoV-2 Spike S1, His-Avi Tag (Cat: bs-46004P), the EC50 is 912.27ng/ml.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
